| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
나노 |
| 제목 |
MMP-2 Proteolytic Cleavage-driven Fluorescence Turn-on of Graphene Oxide Assemblies |
| 초록 |
Altering the properties of nanomaterials through molecular arrangement is of great interest in the field of biosensing. Especially, molecule-induced assembly of nanomaterials allows the effective modulation of their properties, which can be a promising approach for sensing. Herein, we present a graphene oxide (GO)-based optical ‘turn-on’ biosensor for the detection of MMP-2. Fluorescent GO was functionalized with MMP-2 specific peptide substrate containing sulfhydryl group. The cross-linking between GO-Peptide caused by disulfide bonds leads to the assembly of GO followed by self-quenching its inherent fluorescence. This peptide-induced GO assembly is then disassembled by proteolytic cleavage in the presence of MMP-2, thereby restoring the self-quenched GO fluorescence. With this approach, we were able to detect MMP-2 and to investigate its response kinetic. The GO-peptide assembly was successfully applied to the sensitive detection of MMP-2 secreted by living cells. |
| 저자 |
양진경1, 전수지1, 주종민1, 김혜인1, 이윤식2, 김종호1
|
| 소속 |
1한양대, 2서울대 |
| 키워드 |
Graphene oxide; Assembly; Self-quenching; Turn-on sensor; MMP-2
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| E-Mail |
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