| 학회 |
한국고분자학회 |
| 학술대회 |
2017년 가을 (10/11 ~ 10/13, 제주컨벤션센터) |
| 권호 |
42권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
Clustering of CTLA-4 siRNA and CPG oligodeoxynucleotide encapsulated gold nanoparticles for tumor targeting and immunotherapy |
| 초록 |
Small size and highly cationic gold nanoparticles (AuNPs) loaded with CTLA-4 siRNA, CPG ODN, and targeting moieties are assembled to clusters by click chemistry using an alkyne-MMP-2 cleavable peptide as a linker for enhanced tumor targeting and immunotherapy. Branched PEI (bPEI, Mw 10 k) is thiolated by substituting the primary amines. To conjugate azido groups on HS-bPEI for click chemistry, maleimide-PEG-NHS is first reacted with 3-azido-1-propanamine, and then reacted with HS-bPEI. HS-bPEI and HS-bPEI-PEG-N3 are mixed with chlorocauric acid solution and sodium borohydride dissolved in cold water is then rapidly added for synthesis of AuNPs. The AuNPs are highly cationically charged with abundant amine groups and displays azido groups on the surface, and are thus suitable for encapsulation of siRNA, conjugation of targeting antibodies and clustering. The digested AuNPs are expected to be easily internalized by immune cells in tumor site, and facilitate tumor immunotherapy. |
| 저자 |
모미, 손영주, 이솔, 신지운, 이주원, 송아현, 유혁상
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| 소속 |
강원대 |
| 키워드 |
gold; nanoparticle; siRNA
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| E-Mail |
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