| 초록 |
We developed a novel method for the efficient capture of waterborne bacteria by creating an autonomous internal reflux of the magnetic nanoparticle chains (MNCs) inside a flow channel. A glass tube containing positively charged polyethyleneimine-coated MNCs (PEI-MNCs) was placed at the center of a Halbach ring, generating a strong and uniform magnetic field inside the ring. A k0 ring was placed behind the Halbach ring. The k0 ring was used to apply a magnetic force in the opposite direction of the flow and create an autonomous reflux of the PEI-MNCs inside the tube, reducing their loss and increasing their capture efficiency. The capture efficiency of Escherichia coli (E. coli) O157 was greater than 90% at a flow rate of 15 mL/min. E. coli O157 was detected using qPCR, and the limits of detection were 2 and 3 cfu/mL in deionized water and river water, respectively. |
