| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 가을 (10/25 ~ 10/27, 대전컨벤션센터) |
| 권호 |
23권 2호, p.1698 |
| 발표분야 |
생물화공 |
| 제목 |
A programmable static droplet array for monitoring cell-cell communication in a confined microenvironment |
| 초록 |
We present a programmable static droplet array (SDA) for in-situ analysis of the effect of population on natural cell-cell contact and signaling processes in a confined microenvironment. This SDA enables user-demanded unit operations by trapping, encapsulation, arraying, storage, and incubation of individually addressed droplet. The main advantages of programmability, addressability, and selective recovery of droplet open general screening platform. We monitored the response of budding yeast to mating pheromones, as an example of eukaryotic cell-cell communication, with this SDA device. We found morphological and doubling-time changes during the mating reaction with a higher accuracy. Further, the SDA is capable of detecting the function of genes in yeast mutants defective for different aspects of pheromone signaling. |
| 저자 |
진시형1, 이성식2, Matthias Peter3, 이창수4
|
| 소속 |
1충남대, 2Institute of Biochemistry, 3ETH Zürich / Scientific Center for Optical and Electron Microscopy (ScopeM), 4ETH Zürich |
| 키워드 |
생물화공 |
| E-Mail |
|
| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
