| 초록 |
Abundant and cheap carbon sources can effectively reduce the production cost and enhance the economic feasibility. Previously conversion of acetate into itaconate was conducted with rational metabolic engineering strategy, proving the potential of acetate as a promising carbon source. In this study, we applied combinatorial approach to further enhance the production from acetate. Initially, the itaconate-specific screening device was constructed using recently characterized transcriptional regulator. The selective growth was validated in accordance with the addition of itaconate. Thereafter, Adaptive Laboratory Evolution was repeated over several rounds and the increase in growth rate was observed under the selection pressure. The evolved strain could produce 1.06 g/L of itaconate, a 1.24-fold increase in comparison with parental strain and the yield could be significantly enhanced by 7.16-fold. |
