| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생물공학 |
| 제목 |
Optimization of Enzymo-Chemical Screening Method for Engineering Soluble Methane Monooxygenase (sMMO) for Enhancing Catalytic Activity toward Methane |
| 초록 |
Methane is a valuable resource in itself. But the resource has some issues hard to use. It exists as a gas making it expensive to transport to remote locations. Another issue is that methane is a potent greenhouse gas. It has global warming potential more than 20 times that of CO2. Methane monooxygenases (MMOs) are enzymes that catalyze of methane to methanol in methanotrophic bacteria. Through these enzymes, methane can be selectively converted to methanol in mild condition. But the conversion rate of the enzymes are pretty low. So they should be engineered to satisfy an industrially viable level. The inability to express MMOH (Hydroxylase) in a heterologous host limits their utilization in engineering. In our work, to manipulate sMMOH from Methylosinus trichosporium OB3b in E.coli strains, we chose a method called cell-free protein synthesis. And then we focused on optimizing enzymatic assay for screening to measure concentration of methanol which is converted by sMMO clones. |
| 저자 |
박예섭1, 임혜진2, 김동명2, 유태현1
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| 소속 |
1아주대, 2충남대 |
| 키워드 |
Enzyme engineering; Cell free protein synthesis; Methane monooxygenase
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| E-Mail |
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