| 학회 |
한국화학공학회 |
| 학술대회 |
2020년 가을 (10/14 ~ 10/16, e-컨퍼런스) |
| 권호 |
26권 1호, p.301 |
| 발표분야 |
생물화공 |
| 제목 |
Genomic and metabolic analyses of microbial cellulose producer |
| 초록 |
Bacterial cellulose nanofiber (CNF) is a polymer with a wide range of potential industrial applications. Several Komagataeibacter species, including Komagataeibacter xylinus as a model organism, produce CNF. However, industrial application of CNF has been hampered due to inefficient CNF production. Here, we present complete genome sequence and a genome-scale metabolic model (GEM) of K. xylinus DSM 2325 for metabolic engineering applications. To understand the metabolic characteristics of K. xylinus, its GEM KxyMBEL1810 was reconstructed using genome annotation. Random flux sampling analysis was used to predict gene overexpression targets, pgi and gnd genes, for the enhanced CNF production. This prediction was experimentally validated by individually introducing heterologous pgi and gnd genes. Batch fermentation of engineered K. xylinus overexpressing the E. coli pgi gene produced 3.15 g/L of CNF in a complex medium containing glucose, which was 115.5% higher than that (1.46 g/L) obtained with the control strain. These results should be useful resources for metabolic engineering of K. xylinus for the CNF production [Funding information: Samsung Advanced Institute of Technology]. |
| 저자 |
장우대1, 김태용2, 김현욱1, 심우용2, 류재용1, 박진환2, 이상엽1
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| 소속 |
1KAIST, 2Samsung Advanced Institute of Technology |
| 키워드 |
바이오소재; 생물공정
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| E-Mail |
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| 원문파일 |
초록 보기 |
