| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 봄 (04/09 ~ 04/10, 고려대학교) |
| 권호 | 29권 1호, p.18 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Photo-regulation of DNA Functions by Azobenzene-tethered DNA |
| 초록 | DNA has not only a biological role of storing genetic information but also various physical and chemical functions. These role and functions are mainly based on the spontaneous hybridization of two strands that are complementary each other. If we can photo-regulate formation and dissociation of the duplex as shown in Fig.1, scope of the application should be extended, such as artificial regulation of gene expression, DNA {image1} computer, molecular machine, and so on. For this purpose, we have synthesized modified DNA tethering azobenzenes, and hybridization with its complementary strand has been successfully photo-regulated. Here, efficient photo-switching of hybridization with this modified DNA and its application to the bio-reaction are introduced. Modified DNA (Azo-9 in Fig.1) tethering nine azobenzene residues is synthesized from its corresponding phosphoramidite monomer together with the conventional ones (A, G, C, T) by use of DNA synthesizer. The melting temperature (Tm) of the duplex between Azo-9 and its complementary C significantly changes either by irradiating UV or visible light: Tm is 57.6 ℃ when the azobenzene residues take trans-form. However, trans → cis isomerization by UV light irradiation (300 nm < λ < 400 nm) makes Tm drop to 28.9 ℃. The change of Tm induced by photo-irradiation is as large as 28.7 ℃. The isomerization is completely reversible so that the cis-azobenzene is again isomerized to trans-form by visible light irradiation (400 nm < λ). Concurrently, Tm elevates to 57.6 ℃. Thus, formation and dissociation of DNA duplex can be reversibly photo-regulated only by light irradiation without changing pH, ionic strength, and so on. On the basis of this phenomenon, photo-switching of various enzymatic reactions is also achieved. |
| 저자 | Hiroyuki Asanuma |
| 소속 | Research Center for Advanced Science and Technology |
| 키워드 | |
