| 학회 |
한국고분자학회 |
| 학술대회 |
2016년 봄 (04/06 ~ 04/08, 대전컨벤션센터) |
| 권호 |
41권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
Temperature-mediated elasticity modulation of fibroblast-derived matrix induces control of stem cell fate |
| 초록 |
Biophysical properties of the microenvironment, including matrix elasticity and topography, are known to affect various cell behaviors; however, the specific role of each factor is unclear. In this study, fibroblast-derived matrix (FDM) was used as cell culture substrate and physically modified to investigate the influence of its biophysical properties changes on human umbilical vein endothelial cells (HUVECs) and human mysenchymal stem cells (hMSCs) behaviors in vitro. These FDMs were physically modified by simply storing them at different temperatures: the one stored at 4 °C maintained its original properties was considered as natural FDM, whereas the ones stored at -20 or -80 °C exhibited a distinct surface morphology were considered as physically modified FDM. Physical modification induced matrix fiber re-arrangement in FDM, forming different microstructures on the surface as characterized by focused ion beam (FIB)-cryoSEM. A significant increase of matrix elasticity was found with physically modified FDMs as determined by atomic force microscopy (AFM). HUVECs and hMSCs behaviors on these natural and physically modified FDM were observed and compared with each other and with gelatin-coated coverslips. HUVECs showed a similar adhesion level on these substrates at 3 h, but exhibited different proliferation rates and morphologies at 24 h; HUVECs on natural FDM proliferated relatively slower and assembled to capillary-like structures (CLSs). It is observed that HUVECs assembled to CLSs on natural FDMs is independent on the exogenous growth factors and yet dependent on nonmuscle myosin II activity. This result indicates the important role of matrix mechanical properties in regulating HUVECs vascular morphogenesis. As for hMSCs multilineage differentiation, adipogenesis is improved on natural FDM that with lower matrix elasticity, while osteogenesis is accelarated on physically modified FDMs that with higher matrix elasticity, these results further confirms the crucial role of matrix elasticity on cell fate determination. |
| 저자 |
Ping Du1, Muhammad Suhaeri1, Ramesh Subbiah1, 반세영1, 박지민1, 김상헌1, 박귀덕1, 이강원2
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| 소속 |
1한국과학기술(연), 2서울대 |
| 키워드 |
Extracellular matrix (ECM); fibroblast-derived matrix (FDM); physical modification; matrix elasticity; vascular morphogenesis; multilineage differentiation
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| E-Mail |
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