| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-콜로이드·계면화학 |
| 제목 |
High performance multiplex detection of SNPs by using encoded hydrogel micro particles with universal mismatch DNA probes. |
| 초록 |
Graphically encoded hydrogel micro-particles can be utilized to detect multi target biomarkers. A particular area of interest is single-nucleotide polymorphism (SNP) sensing. Hydrogel microparticles that utilize probe-target hybridization as a key mechanism are hampered by the small duplex stability differences resulting from single base-pair mismatches. We have developed encoded hydrogel microparticles with universal mismatch DNA probes for multiplex SNP detection. A widely used base analog (5-nitroindole) was used to assess the ability of the probe to detect a mutation at a given SNP site. We certified that our hydrogel microparticles with DNA probes where one base is substituted with 5-nitroindole exhibit much higher specificity for a single base-pair mismatch with minimal reduction in sensitivity. Our particles can also detect multiple SNPs located in different target strands. |
| 저자 |
문현준, 문석준, 이준호, 노윤호, 임용준, 최준희, 봉기완
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| 소속 |
고려대 |
| 키워드 |
Encoded Hydrogel Microparticles; Single Nucleotide polymorphism; Nucleic acids detection; Multiplex detection
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| E-Mail |
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