| 학회 | 한국공업화학회 |
| 학술대회 | 2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 | 25권 2호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | Multiplex and sensitive SNP genotyping assay with ligase-detection reaction coupled CE-SSCP |
| 초록 | Accuracy, simplicity, sensitivity, quantification and cost-effectiveness are the most important criteria for a genotyping method for single-nucleotide polymorphisms (SNP). One method developed for SNP genotyping, ligase-based coupled capillary electrophoresis (CE) is considered for clinical diagnosis. However, conventional CE system is the design process and multiplex assay procedure are complicated because of the DNA size-based separation principle. In this study, we developed a robust multiplex genotyping method using ligase detection reaction and high-resolution CE-based single-strand conformation polymorphism (CE-SSCP). With this high-resolution CE-SSCP system, we were able to use similar-sized probes, simplifying the design step and assay process. And we found that this method could accurately discriminate single base mismatches and quantifying analysis of SNPs of tp53 gene used as targets for multiplex detection using multi-color fluorescence labeled probes. |
| 저자 | 성준영, 신기영, 정규열 |
| 소속 | POSTECH |
| 키워드 | CE-SSCP |
