| 초록 |
Lysosome, which is able to kill foreign substances in human body, cannot degrade the bacteria cell if the functional group of the cell wall is converted from N-acetylation to O-acylation. It means that specific functional groups on lysosomal membrane would affect the antimicrobial activity of lysosomes after recognizing invader microorganisms. Therefore, we have analyzed the surface modification of lysosomal membrane treated by H2O2 as an activator and NH4Cl as an inhibitor, using X-ray photoelectron spectroscopy. When lysosome was treated by H2O2, the C1s peak were increased C-O and C-N component with binding energies of 286.7 and 285.0 eV in accordance with decreasing O-C=O component 288.1 with binding energies. On the other hand, the P2p peak at lysosome treated by NH4Cl were increased P2O5 or PO4 with binding energy of 133.7 eV. Therefore, we found that some functional groups on the surface of lysosomal membrane were differentially modified by chemical stimulation. |
