| 초록 |
1,3-Propanediol (1,3-PDO) is a commercially valuable platform compound. Its production from glucose by Escherichia coli has been commercialized by DuPont, but no details are not known. In this study, E. coli BL21(DE3) was engineered to have two synthetic pathways, the one converting the glycoytic intermediate dihydroxyacetone phosphate (DHAP) to glycerol; and the other converting glycerol to 1,3-PDO. In addition, the pathway used for assimilatory utilization of glycerol as carbon source was disrupted. Furthermore, to enhance intracellular NAD(P)H availability, the three glucose metabolic pathways (Embden-Meyerhof-Parnas, pentose phosphate, and Entner-Doudoroff) were modified. The engineered strain could successfully produce 1,3-PDO when grown on glucose. Further study to improve 1,3-PDO yield is under progress. |
