| 학회 |
한국고분자학회 |
| 학술대회 |
2017년 가을 (10/11 ~ 10/13, 제주컨벤션센터) |
| 권호 |
42권 2호 |
| 발표분야 |
대학원생 구두발표(영어발표, 발표15분) |
| 제목 |
A trilayer structured nanofilm with efficient encapsulation and sustained release of bFGF to extend medium replacement cycle in human iPS culture |
| 초록 |
Induced pluripotent stem cells (iPSCs) have the potential to generate patient-specific regenerative medicine. Especially, basic fibroblast growth factor (bFGF) is essential signaling molecules to maintain undifferentiated state and pluripotency of iPSCs. However, the bFGF should be daily supplied to iPSCs culture medium due to its instability in physiological condition. So, we established the nanofilm to maintain the bFGF level in iPSCs culture medium. This nanofilm has layer-by-layer assembled multi-trilayer structure, which provides attractive advantages such as enhanced encapsulation efficiency of bFGF and short build-up time compared with conventional multilayer nanofilm. As a result, the release amount of bFGF from the nanofilm was 50.7ng/cm2 during 3 days. Also, the iPSCs were grown in the presence of the nano-film maintained their undifferentiated morphology and expression levels of pluripotency marker proteins despite of tri-daily replacement of growth medium. |
| 저자 |
한의영1, 홍진기1, 박희호2, 박태현2, 박주현3
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| 소속 |
1중앙대, 2서울대, 3강원대 |
| 키워드 |
controlled release; basic fibroblast growth factor; induced pluripotent stem cell; multi-trilayer film; layer-by-layer assembly
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| E-Mail |
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