| 초록 |
In this study, three elementary schools located near roads with heavy traffic in Seoul were selected. Bioaerosols were collected in classrooms and outdoors using Polyvinyl chloride (PVC) filters and air pumps to understand complex mechanisms of microbe. Next-generation sequencing (NGS) and quantitative Polymerase Chain Reaction (qPCR) were used to analyze all microorganisms present in the environment. Microbial communities of classrooms and outdoors were analyzed by targeting bacterial 16S rRNA genes and fungal Internally Transcribed Spacer (ITS) regions. Bacteria such as Enhydrobacter, Micrococcus, and Staphylococcus relate to humans were found in great abundance in the classroom air. For fungi, Cladosporium, Clitocybe, and Daedaleopsis were abundant genera and mostly related to plants. The main source of airborne bacteria in the classrooms is humans. However, most of the fungi floating in the classroom air originate outdoors. |
