| 학회 |
한국고분자학회 |
| 학술대회 |
2012년 봄 (04/12 ~ 04/13, 대전컨벤션센터) |
| 권호 |
37권 1호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Ni2+ Complexed Nitrilotriacetic acid-End-Functionalized Poly[(poly ethylene glycol) ethyl ether methacrylate]; Stabilization of Histidine-tagged Green Fluorescent Proteins in Organic Environment. |
| 초록 |
Non-covalent method was used for bioconjugation of Ni2+ complexed nitrilotriacetic acid (NTA) end-functionalized polymers with genetically modified six histidine-tagged green fluorescent proteins (His6-GFP). NTA end-functionalized poly[poly(ethyleneglycol) methyl ether methacrylate] (NTA-p(PEGMA)) was prepared using initiators containing tert-butyl protected NTA moiety via atom transfer radical polymerization (ATRP). Well-defined NTA-p(PEGMA) was characterized using 1H NMR, and GPC. Using end-functionalized polymers with amphiphilic structures, stabilization of proteins was attempted due to the formation of micelles in the solution. D-values for the stabilization of protein by conjugation with end-functionalized polymer were determined using fluorescent spectroscopy. The micelles of conjugates were observed using transmission electron microscopy (TEM) observations and dynamic light scattering (DLS). |
| 저자 |
김봉수1, 백현종1, 하은주1, 정소미1, 이선구1, 안승수2
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| 소속 |
1부산대, 2경원대 |
| 키워드 |
ATRP; nitrilotriacetic acid end-functionalized poly(PEGMA); histidine-tagged GFP; protein stability; bioconjugation
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| E-Mail |
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