| 초록 |
A biodegradable polymer and biomaterial blending is one of the most effective methods for tissue engineering. The polymer scaffolds were fabricated by mixing of poly ε-caprolactone(PCL)/gelatin/DMEM[PGD] and after then PGD scaffolds were plasmatreated by O2 reactive gas(O-PGD). Chemical and physical properties of PGD were characterized by biodegradability test, FTIR, tensile strength and contact angle. The hydrophilic properties of the PGD surface were improved by the addition of gelatin, DMEM and atmosphere O2 plasma treatment. The results showed that these biomaterials were found to support cell growth. MTT assay, cell attachment efficiency, SEM and confocal microscope results showed that the PG, PGD and O-PGD scaffolds enhanced the cell attachment and growth compared to PCL nanofibers. Importantly, cell attachment and growth on PGD and O-PGD show notable improvement over those on PCL and PG scaffolds. The PGD and O-PGD are considered to be useful as cell supporting biomaterials. |
