| 초록 |
Nitric oxide (NO) delivery has been actively studied in chemical and engineering fields since it was discovered in mammals as an essential cell signaling molecule which is concerned with various physiological functions. Notably, all functions of NO depend on its concentration, time, and applied site. Thus, controlling release amount/rate and targeting of NO is important for biomedical applications. In this study, we developed NO-releasing nanoparticles (NPs) by a straightforward method. We coated silica NPs with branched polyethylene imine (BPEI) via electrostatic interaction and amine groups of BPEI were converted to chemical NO donors under high pressure NO gas. Developed BPEI-coated NO-releasing NPs (BPEI-NO NPs) spontaneously released 3.5 umol/mg of NO during a day. For in vitro studies, we investigated non-cytotoxicity of BPEI-NO NPs to cells and bactericidal effects with the range up to 500 ug/mL. For in vivo study, we examined the ocular wound healing efficiency of BPEI-NO NPs. |
