| 초록 |
Three-dimensional (3D) cell culture platforms have drawn attention since the 3D cell culture is able to recapitulate in vivo microenvironment of tissues. Herein, we suggest novel elastomeric cavity structures with controllable sizes and curvatures for spheroid formation as tunable 3D cell culture system. In this platform, cavities are fabricated by one-step spontaneous interfacial reaction between water droplets and immiscible liquid polydimethylsiloxane. Using optimized cavities, single tumor spheroids are successfully formed in each cavity with high efficiency. We formed tumor spheroids with fibroblasts to reflect microenvironment of tumor tissue. For further application, this platform can be directly used for biological analyses such as drug screening and optical monitoring using tumor spheroids. We believe that our proposed platform would significantly contribute to tissue engineering and cancer research. |
