| 초록 |
The field of research for an adequate delivery system for DNA and RNA delivery has been a 'hot pot' for several years. Nucleotide delivery systems for gene therapy may be classified into viral and non-viral vector systems. Of these, nanoparticle mediated non-viral gene delivery vectors go through the endocytosis process when the nanoparticles deposit into cells. However, difficulties occurring from the low efficiency of endosomal escape of the nanoparticles is a major hurdle in gene delivery for all kinds of delivery cargos. If a DNA encapsulated nanoparticle fails to escape the endosome, the cargo goes through a degradation process by lysosomes and low drug delivery rate comes as a consequence. Therefore, techniques to overcome endosomal digestion is a key to successful development of DNA delivery systems. Studies using cell penetrating peptide (CPP) or osmotic pressure from ionic concentration gradation are being conducted as a breakthrough. |
