| 학회 |
한국재료학회 |
| 학술대회 |
2012년 가을 (11/07 ~ 11/09, 라카이샌드파인 리조트) |
| 권호 |
18권 2호 |
| 발표분야 |
D. 바이오재료(Biomaterials) |
| 제목 |
Bone regeneration in rat calvaria defect using porous PCL/BCP scaffolds with surface modification |
| 초록 |
To make polycaprolactone (PCL) more suitable for bone tissue engineering, PCL in the form of porous scaffolds was first loaded with biphasic calcium phosphate (BCP) and then modified with 1,6-hexamethylenediamine to introduce amino groups on their surface. Collagen was then coated on their surface, and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide used as the crosslinker agent. The appropriateness of these porous PCL and PCL-BCP scaffolds for the tissue/cell culture was evaluated in vitro with mouse calvaria-derived preosteoblastic cells (MC3T3-E1). The surface modification of PCL and PCL-BCP scaffolds with collagen showed the good ability to support the attachment, proliferation and protein expression, mRNA expression of the pre-osteoblastcells. The in-vivo study was done by implanting the porous PCL, PCL-Collagen, PCL-BCP and PCL-BCP-Collagen to the skull of rats. Newly bone formed in PCL-BCP-Collagen after 6 weeks implantation were observed by hematoxylin&eosin staining. The PCL-BCP-Collagen hold a potential candidate as scaffolds for the Osteoconduction of bone regeneration. |
| 저자 |
Ji-Hyun Kim1, Nguyen Thuy Ba Linh2, Young Ki Min3, Hun-Mo Yang4, Byong-Taek Lee2
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| 소속 |
1Department of Biomedical Engineering & Materials, 2College of Medicine, 3Soonchunhyang Univ., 4Department of Biomedical Engineering and Materials |
| 키워드 |
PCL; BCP; Porous scaffold; aminolysis; bone regeneration
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| E-Mail |
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