| 초록 |
Therapeutic gene indicates short half life and low stability due to denaturation. Therefore, the therapeutic gene is complexed with non-viral vectors such as cationic polymers or lipids. In this study, cationic polymer was used as non-viral vector, and complexed with therapeutic gene (polyplex). Non-viral vectors are safer than viral vectors however there are many obstacles that non-viral vectors face long delivery pathways. To overcome the limitation of gene therapy with non-viral vectors, we used human mesenchymal stem cells (hMSCs) to target tumor and photochemical internalization (PCI) to enhance transfection efficiency. We demonstrate that therapeutic gene loaded hMSCs (polyplex@hMSCs) facilitate tumor targeting and PCI could increase the expression of therapeutic gene in polyplex@hMSCs. All the taken together, PCI-mediated polyplex@hMSCs (PCI-polyplex@hMSCs) offer exciting potential therapeutic effect in cancer gene therapy. |
