| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 봄 (04/07 ~ 04/08, 대전컨벤션센터) |
| 권호 |
36권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Stimuli-sensitive copolymer nanocapsules for nontoxic and efficient non-viral gene delivery |
| 초록 |
We synthesized a new biodegradable pentablock copolymer poly(β-aminoester)/poly(ε-caprolactone)/poly(ethyleneglycol)/poly(ε-caprolactone)/poly(β-aminoester)(PAE-PCL-PEG-PCL-PAE) with a pH-responsive unit (PAE blocks) and used as a nontoxic gene carrier. At low pH (pH＜6.0), the copolymer is able to interact with plasmid DNA to form polyelectrolyte complexes. Transition to neutral pH causes the deprotonation of the PAE blocks, leading to the formation of water-insoluble shells consisting of the PCL and PAE blocks. At the same time, the plasmid DNA can be localized in free form within the nano-sized capsule-like structure. The hydrophilic PEG segment may greatly improve the solubility and blood-serum compatibility of the nanocapsules. In addition, the unprotonated tertiary amines of the PAE blocks can facilitate the endosomal escape of the nanocapsules. This study assessed the possibility of using nanocapsules as a safe and efficient gene carrier for systemic gene therapy. |
| 저자 |
이민상1, 장연림1, 김명구1, Dai Phu Huynh1, Cong Truc Huynh1, 이유한2, 채수영1, 김선화3, 박태관2, 이두성1, 정지훈1
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| 소속 |
1성균관대, 2한국과학기술원, 3연세대 |
| 키워드 |
gene delivery; copolymer
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| E-Mail |
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