| 초록 |
Graphene oxide (GO) has recently attracted great attention as a new type of fluorophore. This 2-dimensional carbon sheet obtained from oxidation of graphene has not only good biocompatibility but more stable fluorescence in physiological conditions than conventional organic fluorophores. In addition, aggregation of GO is often accompanied by fluorescence self-quenching, which can provide a basis for fluorescent sensing. Here, we present a GO-peptide optical sensor, which can detect MMP-2 based on disulfide bond triggered assembly/target specific disassembly. The cross-linking between GO-peptide sheets mediated by disulfide bonds leads to GO-peptide assembly, which decreases its inherent fluorescence via self-quenching and lowers the concentration of fluorophores. GO-peptide assembly is dissociated through proteolytic hydrolysis of the peptide in the presence of MMP-2, restoring its quenched fluorescence. This sensing platform is simple and highly sensitive to detect MMP-2 (~2 ng mL-1 level), secreted from living cells. |
