| 초록 |
This study was aimed to enhance polycyclic aromatic hydrocarbon(PAHs) biodegradation rate by repeated-batch treatment using immobilized cells of Phanerochaete chrysosporium. In the repeated-batch operations with 30 mg/L of pyrene, the maximum degradation rate was 6.58 mg/L·day. As the number of batches increased, the concentration of immobilized cells significantly decreased, and the degradation rate and specific activity gradually increased to a maximum value and then decreased. To have PAH degradation activity and cell mass recovered, one batch of cultivation using the growth medium instead of the PAH-degrading medium was carried in the course of repeated-batch operations. This led to a significantly recovered cell mass and degradation activity although the original levels were not achieved. In the repeated-batch operations for degrading anthracene, a similar trend was observed, where the maximum degradation rate was 5.36mg/L·day. In the repeated-batch operations for simultaneous degrading anthracene and pyrene, the degradation rates were lower than those in separate treatments. The maximum degradation rates of pyrene and anthracene were 4.29 and 4.46 mg/L·day, respectively. Overall, the rate of PAH degradation could be enhanced 2.5-3.0 folds by using immobilized cells compared to the case of using suspended cells. |
