| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 |
16권 2호, p.1531 |
| 발표분야 |
생물화공 |
| 제목 |
P450 mediated hydroxylation of phloretin using Streptomyces avermitilis |
| 초록 |
Twelve microorganisms were initially screened for their abilities to catalyze biotransformation of phloretin. Streptomyces avermitilis, two main products were identified in GC/MS analysis. They were interpreted as hydroxylated products of phloretin in A-ring at different position. (mass increase 179 → 267,192 → 280) This result confirmed hydroxylaion considering BSTFA derivatization of hydroxylated product. Maximum conversion was 6.7 %, which was achieved for 1 hours of reaction, and the substrate (phloretin) and reaction product was completely metabolized after 3 hours of reaction. Three kinds of Cytochrome P450 inhibitor, Coumarin, Erythromycin and Quinidine was added with 0.5mM final concentration to find out the role of P450 enzymes. P450 amplifeid and deleted Streptomyces were also used to confirm the role of P450 enzyme. |
| 저자 |
김우일1, 이창수1, 박종화1, 김준형1, 김병기2
|
| 소속 |
1동아대, 2서울대 |
| 키워드 |
Flavonoid; hydroxylation; Streptomyces Avermitilis; biotransformation
|
| E-Mail |
|
| 원문파일 |
초록 보기 |
