| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Novel high-throughput screening technology for microalgae with high hotosynthetic efficiency |
| 초록 |
Photosynthetic organisms evolved phototaxis to find optimal light condition for optimal photosynthesis. Here we report rapid screening system using phototaxis of Chlamydomonas reinhardtii to isolate strains with enhanced photosynthetic efficiencies in microfluidic system. We proved the correlation between phototaxis and PSII efficiency by quantitative analysis of phototaxis. We enriched the strains with improved PSII efficiency by isolating cells showing fast phototactic response from 10,000 mutant strains. Among 147 strains isolated after screening 94.6% showed enhanced growth over the parental strain. Two mutants showed much improved performances with up to 1.9- and 8.1-fold increases in growth and lipid production, respectively. Our approach provides a powerful screening tool for rapid improvement of microalgae to enhance photosynthetic efficiency. |
| 저자 |
PHAM HOANG MINH, 곽호석, 최홍일, 성영준, 심상준
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| 소속 |
고려대 |
| 키워드 |
Chlamydomonas reinhardtii; phototaxis; high-throughput screening; mutant
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| E-Mail |
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