| 학회 |
한국화학공학회 |
| 학술대회 |
2016년 가을 (10/19 ~ 10/21, 대전컨벤션센터) |
| 권호 |
22권 2호, p.1819 |
| 발표분야 |
생물화공 |
| 제목 |
Expression and characterization of recombinant Cellulomonas flavigena Endo-1,4-β-xylanase in Pichia pastoris |
| 초록 |
Xylan is a major component in plat cell wall and the second abundant polysaccharides in nature. Endo-1,4-β-xylanase (XYN) is the most important xylan degrading enzyme, which cleaves the β-1,4-glycosidic bonds in xylan backbone and has various applications in industries. In this study, a gene encoding xyn from Cellulomonas flavigena has been cloned into pPICZα B as expression plasmid and was expressed in the heterologous host Pichia pastoris, under the control of the AOX1 methanol inducible promoter. The correct integration in the Pichia genome was confirmed by PCR with the XYN forward and the 5’ AOX1 reverse primers. The production of recombinant xylanase (rXYN) was carried out with specific media for 96 h. The biochemical characterization of rXYN was investigated under pH 4-9 and temperature of 30-65℃. As a result, the optimal pH and temperature were determined as pH 6.0 and 55℃, respectively. Finally, the specific activity of rXYN was about 4,400 U/mg–protein under the determined conditions. |
| 저자 |
양지현, 김찬겸, 김동섭, 이주훈, 이수권, 김승욱
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| 소속 |
고려대 |
| 키워드 |
미생물공학 |
| E-Mail |
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| 원문파일 |
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