| 초록 |
Gelatin and poly (vinyl alcohol) (PVA) were used as extracellular matrices (ECM) in the preparation of encapsulated hepatocytes using alginate and barium chloride. The macroporous capsules membranes were characterized by FTIR to verify the polyion complex formation, XRD to observe the effect of blending on crystallinity, DSC and TGA to assess the thermal stability. From microscopic observation, it revealed that the microcapsule has a highly porous structure with interconnected pores. The permeability of microcapsules was investigated using proteins with different molecular weight (Mw=15 kDa ~ Mw=150 kDa) and the molecular weight cut-off (MWCO) of capsules was 150 kDa, showing the potential that it can function as an immunoprotecting wall. Moreover, the hepatocytes cultured with gelatin and PVA in the core of the microcapsules, rapidly aggregated within a day, thus resulting in good metabolic functions such as ammonia removal and albumin synthesis. |
