| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 봄 (04/23 ~ 04/25, 제주ICC) |
| 권호 |
14권 1호, p.314 |
| 발표분야 |
분리기술 |
| 제목 |
Development of AC Microelectrophoresis for Rapid Protein Affinity Evaluation |
| 초록 |
We have developed a new method for evaluating the affinity interactions between two different proteins by applying an alternating current (AC) voltage to a micro-flow channel. An AC voltage was applied to the protein-modified microspheres in the micro-flow channel, which resulted in the oscillation of the microspheres owing to their surface charges. The oscillation amplitude showed a linear relationship with the charge density of the microspheres. As an example for protein-affinity measurement, the amplitude changes of a profilin-modified microsphere were measured by the addition of actin. In the same electrical condition, the oscillation amplitude of the profilin-modified microsphere increased by 175% by binding with actin. Similar results in the principle were obtained for the affinity interaction between biotin and streptavidin. The results showed that the higher the charge density of the microspheres induced by binding with different proteins, the higher the oscillation amplitude of the microspheres, thus, suggesting a possible application of the micro-flow channel and AC voltage on the protein property study, as well as on the biosensor application using the oscillation amplitude changes. |
| 저자 |
김승진1, 신정하1, 이지선1, 김우식2, 장상목1, 김종민1
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| 소속 |
1동아대, 2경희대 |
| 키워드 |
Affinity; Bioprocess Monitoring; Biosensors; Protein; Electrophoresis; AC voltage
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| E-Mail |
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| 원문파일 |
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