| 초록 |
Lactobionic acid (LBA) is a recently emerging chemical which has been widely utilized in various industries. we attempted to produce LBA from lactose in E. coli, which is innately unable to produce LBA, by co-expressing a heterologous quinoprotein glucose dehydrogenase (GDH) gene and pyrroloquinoline quinone (PQQ) synthesis gene cluster. We successfully produced LBA without additional supplementation of PQQ using this recombinant E. coli strain. Changing the type of heterologous GDHs expressed in E. coli was effective to improve the production ability of the E. coli strain. To further enhance the LBA production ability of the E. coli strain, culture conditions were optimized in flask cultures. After then fermentation, this strain showed the higher concentration of LBA production, yield and productivity. Our results provide a simple method to produce LBA from lactose in a naturally non-LBA-producing bacterium and a groundwork for high efficient LBA production in E. coli. |
