| 초록 |
In this study, we propose a mechanically robust lipid nanovesicle system as an effective drug delivery nanocargo during the blood circulation in inflammatory disease. We controlled the rigidity of nanovesicles structured with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) by coassembling with amphiphilic poly(ethylene oxide)-block-poly(ε-caprolactone)-block-poly(ethylene oxide) (PEO-b-PCL-b-PEO). We observed that the presence of PEO end blocks increased the longevity of lipid nanovesicles during blood circulation due to the anti-fouling effect, and the PCL middle block with high crystallinity make the membrane rigid. The stiffness of nanovesicles was analyzed by using quartz crystal microbalance with dissipation (QCM-D). Finally, we demonstrated enhanced blood circulation time of the nanovesicles in the rheumatoid arthritis mice model, suggesting their clinical potential as a therapeutic agent. |
