| 초록 |
It has been found that 2,3-Butanediol is a high-value chemical which can be produced by fermenting waste gas carbon monoxide. However, there are challenges to separate 2,3-BDO because of the hydrophilicity of it. Therefore, conversion of 2,3-butanediol to other hydrophobic products is necessary. In this study, a recombinant cell is constructed, to assist mevalonate production by using 2,3-BDO as a feedstock. The plasmid with the gene which is essential for mevalonate generation was transformed into Pseudomonas putida KT2440. In the experiments on various carbon sources such as 2,3-BDO, glucose, and glycerol, it is confirmed that 2,3-BDO is the most suitable carbon source for mevalonate production. The amount of produced mevalonate under conditions using 2,3-BDO as a carbon source was 1.79 g/L, while those of glucose and glycerol were 0.11 g/L and 0.17 g/L, respectively, for 48 hours. |
