| 초록 |
The Transient Receptor Potential Vanilloid 1 (TRPV1) channel is an appear target for the treatment of pain with a unique expression profile in marginal nociceptors and the ability to show various activation. Several agonists of endogenous and exogenous nature have been described for ion channel. TRPV1 initially identified as the receptor for interaction with capsaicin, temperature greater than 43 °C, acidic conditions and leads to a painful, burning sensation. Based on this, the objective of this study was to test the antigenic potential of the recombinant TRPV1 protein for subsequent use in diagnostic tests. The TRPV1 expressed in the Escherichia coli strain BL21 was conformed from experimentally using ELISA and Western blotting and then the recombinant TRPV1 were applied with the surface of receptor to detect target material and improve sensing performance. |
