| 초록 |
3-HP and 1,3-PDO are useful platform chemicals for further industrial manufacture and applications. It has been reported that the biosynthesis of both chemicals are regulated by internal redox balance (i.e. NAD+/ NADH ratio). In this study, we examined an electrode-based redox regulation of 3-HP and 1,3-PDO production from glycerol using a recombinant E.coli which harbor the metabolic pathways of both chemicals. Our results showed that 3-HP production was enhanced by increasing the intracellular NAD+ / NADH ratio when the oxidation potential was given on the electrode. In contrast, the ratio of NAD+ / NADH was decreased when reduction potential was given, hence the synthesis of 1,3-PDO increased while 3-HP was suppressed. These results indicate that the electrode-based redox control can regulate metabolic pathway of biosynthesis and selectively produce platform chemicals using a bioelectrochemical system. |
