| 초록 |
Equol is a promising isoflavone derivative that mitigates diverse sex hormone related disorders. Recently, we developed a recombinant E. coli strain expressing multiple gut microbial isoflavone reductases, then it displayed much higher equol-producing capacity compared to non-productive natural bacteria. Moreover, engineered equol-producing enzymes with improved biocatalytic performance were rationally designed and exploited for efficient and enantioselective production of (S)-equol and (-)-5-hydroxyequol with high titer (ca. ＞ 1g/L). Meanwhile, we also firstly identified functional oxidases for ortho-hydroxylation of (S)-equol. The oxidases were efficiently utilized in the catalysis of (S)-equol into 6-hydroxy-(S)-equol, 3’-hydroxy-(S)-equol and 6,3’-dihydroxy-(S)-equol. Furthermore, combinatorial use of the equol-producing strain and equol-hydroxylaing strain, enabled efficient and regioselective biosynthesis of ortho-hydroxyequols from soy isoflavone, for the first time. |
