| 초록 |
The viability of tissue-engineered cells is damaged by hypoxia due to rare or no microvessel. Here, we newly developed a spontaneous respiration biomaterial, i.e., Respiratoid, to help cellular respiration so that tissue-engineered cells could survive without microvessel. The Respiratoid could convert carbon dioxide into oxygen during cellular respiration. To this end, the intact chloroplast in plat leaf was introduced to biocompatible alginate via a chloroplast-transit-peptide (CTP) that can ‘anchor’ the outer membrane of chloroplast while maintaining the function of chloroplast. The CTP-conjugated alginate was optimized to make a hydrogel for tissue engineering. After physical characterization of the hydrogel, the cytotoxicity and degree of oxygen/carbon dioxide balance of the Respiratoid were evaluated in vitro and in vivo. Collectively, newly synthesized Respiratoid material could be used for successful tissue engineering. |
