| 초록 |
In this study describes the optimization of culture mediums to improve proteolytic enzyme production of Janthinobacterium sp. using response surface methodology (RSM) and comparative proteomic analysis. Experiments were conducted using one-variable-at-a-time approach, and significant factors affecting proteolytic enzyme production were identified as dextrose, tritone X-100, peptone, and pH. Compared to the original medium, protease activity increased 6.25-fold in optimized medium. Extracellular and intracellular proteins were analyzed by two-dimensional gel electrophoresis to investigate metabolic changes in optimized medium. Some proteins essential to cell viability were highly expressed in the optimized medium, and the outer membrane protein A was secreted at a higher level. These results can be useful for industrial applications of psychrophiles that produce cold-adaptative enzymes. |
