| 초록 |
The main objective in gene therapy is the development of efficient, non-toxic gene carriers that can transport DNA inside the cell nucleus of the target cell. Nonviral vectors have gained a great deal of interest by their advantages of increased safety, reduced immunogenicity and ease of manufacture compared to viral vectors. To develop a biodegradable and efficient gene carriers, we designed monomers to produce cationic poly(carbonate)s that have protonable amine group to condense DNA, secondary or tertiary amine group to provide endosomal buffering effect and biodegradable carbonate backbone linkage. Controls in polymer molecular weight were obtained by ring-opening anionic polymerization. To investigate the increased cellular uptake of DNA/polymer complexes, PEGylated amino acid based cationic copolymers modified with an additional hydrophobic interaction were synthesized by copolymerization of cationic L-lysine and hydrophobic L-phenylalanine. Cytotoxicity ad transfection efficiency were examined for the synthesized PEG-poly(amino acid) copolymers with 20 % phenylalanine contents. |
