| 초록 |
Deoxyribonucleic acid (DNA) has been widely utilized as a versatile building block for the fabrication of various materials. Furthermore, rolling circle amplification (RCA), a powerful technique for continuous replication of DNA, enables us to fabricate macroscopic DNA structures. Here in, we propose two novel biosensors based on RCA for the detection of pathogen DNA. In our biosensor, DNA is continuously amplified from 3´ end of the primer DNA immobilized on the gold nanowire in the presence of the target DNA. As a result of polymerization, macroscopic DNA hydrogel is synthesized which allows us to recognize the existence of the target DNA with naked eye. We also designed another biosensor that DNA polymerization takes place on the top of two separate gold electrodes. After the enzymatic replication of DNA in the presence of the target DNA, amplified DNA successfully covered the gap between the two gold which allow electrical current to flow from one electrode to another. |
