| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 봄 (04/09 ~ 04/10, 대전컨벤션센터) |
| 권호 |
34권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Humoral and cellular immune response in mice injected by polyethylenimine/SARS CoV S DNA vaccine nanoparticles through intranasal route |
| 초록 |
In this study, plasmid pCI-S, encoding the S gene of SARS-CoV was constructed and delivered by polyethylenimine (PEI). The expression of the S protein was confirmed in vitro. The immune responses after intranasal immunization with PEI/pCI-S nanoparticles were examined in BALB/c mice. The mice immunized with PEI/pCI-S nanoparticles produced much higher level of IgG and IgA than those treated with pCI-S alone. Spleens and lungs were isolated from the immunized mice, and then the cells were cultured. The proliferation of B220 cells and some cytokine producing cells (IFN-γ, TNFα, TNFα+ IL-2) isolated from PEI/pCI-S vaccinated mice were increased after restimulated with antigen. Co-stimulatory molecules (CD80 and CD86) and Class II MHC molecules (I-Ad) expressed by dendritic cells also showed higher levels in PEI/pCI-S vaccinated mice than pCI-S vaccinated mice. As the results, the PEI/pCI-S nanoparticles induced both humoral and cellular immune responses. |
| 저자 |
전희선, 심병식, 박성우, 곽정정, 강호림, 윤철희, 조종수
|
| 소속 |
서울대 |
| 키워드 |
SARS CoV S DNA vaccine; polyethylenimine; immune response; BALB/c mice
|
| E-Mail |
|
