| 학회 |
한국고분자학회 |
| 학술대회 |
2019년 봄 (04/10 ~ 04/12, 부산컨벤션센터(BEXCO)) |
| 권호 |
44권 1호 |
| 발표분야 |
콜로이드 및 분자조립 부문위원회 |
| 제목 |
Photostable polymeric nanoparticle containing hydrophobic Lumogen® dye as cellular biomarkers |
| 초록 |
Lumogen® fluorescent dyes have great light stability as well as high fluorescence quantum yields. Despite the excellent photophysical properties of dyes, the highly hydrophobic structure in common makes the compounds difficult to function as biological fluorescence probes. To generally utilize a family of organic fluorescent dyes such as Lumogen® Red, Orange, Yellow, and Violet, as a biomarker for cellular imaging in flow cytometry and fluorescence microscopy, we used an encapsulation method using amphiphilic block copolymer namely Pluronic® F127. The photostability of Lumogen nanoparticles were compared with commercial dye, Alexa488 and DAPI, used primarily bioimaging. Cell uptake of the probe nanoparticles by HeLa cells was analyzed by flow cytometry and confocal laser scanning microscopy. Also, we observed to confirm the position of the Lumogen nanoparticles in the HeLa cells using a high voltage electron microscope. |
| 저자 |
강민경1, 김한성1, 이태형2, 허양훈3, 김영상1, 박선주1, 진준오4, 이창환2, 곽민석1
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| 소속 |
1부경대, 2울산대, 3한국기초과학지원(연), 4영남대 |
| 키워드 |
Polymeric micelle; Lumogen; sIPN; Probe nanoparticle; Cellular uptake
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| E-Mail |
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