| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-콜로이드·계면화학 |
| 제목 |
Highly Specific Multiplex Detection of SNPs via Encoded Hydrogel microparticles conjugated with Universal Mismatch DNA probes |
| 초록 |
Nucleic acid multiplex detection using encoded hydrogel microparticles is a detection technology attracting attention by its high specificity and multiple detection capabilities. In particular, single nucleotide polymorphisms (SNPs), generated by mutation of only one sequence in a nucleic acid, are well known as useful biomarkers. However, encoded hydrogel microparticles that utilize probe-target hybridization as a key mechanism are hampered by the small duplex stability differences resulting from single base-pair mismatches. We have developed encoded hydrogel microparticles with universal mismatch DNA probes for multiplex SNP detection. A widely used base analog (5-nitroindole) was used to assess the ability of the probe to detect a mutation at a given SNP site. We certified that our hydrogel microparticles with DNA probes where one base is substituted with 5-nitroindole exhibit much higher specificity for a single base-pair mismatch with minimal reduction in sensitivity. |
| 저자 |
임용준, 문현준, 문석준, 이준호, 노윤호, 봉기완
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| 소속 |
고려대 |
| 키워드 |
Single Nucleotide polymorphism; SNP; Nucleic acids detection; Multiplex detection; Encoded Hydrogel Microparticles
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| E-Mail |
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