| 초록 |
Single nucleotide polymorphism(SNP) is the most abundant form of variations at a specific location of individual genome. SNPs are important genomic landmarks in disease genetic studies. For this reason, technologies for SNP genotyping have been developed with well-defined solid supporters for enhancing sensitivity and for multiplexing. Specially, DNA-templated silver nanoclusters(DNA-AgNCs) provide a facile route for the generation of hybrid materials that possess sensing capabilities arising from discrete electronic transitions of the AgNC composed of two to tens of silver atoms. The potential of DNA-AgNCs as sensors for nucleic acids is promised by the direct synthesis, water solubility, small size, high photostability, and sequence-dependency. Herein, we report a simple, rapid, homogeneous and label-free method for SNP detection. By modulating the intrinsic fluorescence of DNA-AgNCs in response to target DNA sequences, single-base mismatch detection is achieved within 5 minutes. |
