| 초록 |
A rapid, homogeneous, and in-situ labelable format design to detect single nucleotide polymorphism (SNP) is presented. Fluorescent silver nanoclusters (AgNCs) produced following hydride-mediated reduction of Ag+ bound to a particle double-stranded oligodeoxynucleotide were employed as probes for SNPs. The sensing mechanism is based on the fluorescence enhancement of AgNCs through an irreversible NCs transfer upon hybridization. The “turn on” mechanism by introducing a competitor can be displaced in response to a target DNA. In a controlled system, the fluorescence intensity of AgNCs is ca. 3-fold enhanced upon hybridization with a perfectly matched target DNA, while single-base mismatch detection is achieved within 5 min. Addition of a perfectly matched human aldehyde dehydrogenase 2 (ALDH2) which is utilized as a target for SNP, dramatically enhanced fluorescence intensity is observed (ca. 48-fold) and prompt SNP genotyping is accomplished. |
