| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 봄 (05/02 ~ 05/04, 여수 엑스포 컨벤션) |
| 권호 |
20권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Development of recombinant Pseudomonas denitrificans for enhanced coenzyme B12 production |
| 초록 |
Coenzyme B12 is an essential cofactor for the activity of glycerol dehydratase, an essential enzyme used in 3-HP production. Several recombinant Pseudomonas denitrificans strains have been developed for 3-HP production due to its capability to synthesize coenzyme B12 natively. However, the amount of native coenzyme B12 synthesized was not sufficient for glycerol dehydratase reaction in maintaining high 3-HP production rate. Genes related to Coenzyme B12 pathway are transcriptionally and/or translationally regulated by riboswitches (RS). The riboswitch mutant strains (mutation of RS2,3 and RS4) developed by homologous recombination could not show improvement in either coenzyme B12 synthesis or 3-HP production. RS4 mutant showed deteriorated B12 synthesis and sequentially led to decreased 3-HP production (13.1 mM at 35 h). Characterization and engineering these intergenic regions containing riboswitches has been studied to improve coenzyme B12 synthesis. |
| 저자 |
NGUYEN VO PHU THUAN, Satish Kumar Ainala, 박성훈
|
| 소속 |
Pusan National Univ. |
| 키워드 |
3-HP; coenzyme B12; riboswitch; regulation
|
| E-Mail |
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