화학공학소재연구정보센터
학회 한국고분자학회
학술대회 2019년 가을 (10/09 ~ 10/11, 제주컨벤션센터)
권호 44권 2호
발표분야 기능성 고분자
제목 Inhibitory Effects on Melanin Production in B16 Melanoma Cells of fractions from Schisandra chinensis
초록 Melanin synthesis is controlled by a cascade of enzymatic reactions regulated at the level of tyrosinase in melanocytes and melanoma cells. In an ongoing search for new tyrosinase inhibitors from natural sources, the dried leaves of Schisandra chinensis (SCL) were extracted with 70% acetone and separated into fractions 1-3 (Fr.1-3). Fr.2 showed potent tyrosinase activities with 22.0 % inhibition concentration 1000 µg/mL compared to the positive control, albutin (24.8%). Fr.2 which showed a significant inhibition effect against tyrosinase led to the isolation and identification of three known phenoilic compounds (1-3): chlorogenic acid (1), isoquercitrin (2), quercetin (3). We investigated the changes in protein level of tyrosinase, Melanocyte Inducing Transcription Factor (MITF) by using western blotting. Fr.2 was able to significantly inhibit tyrosinase and MITF expression in protein level.
저자 김한혁1, 정아름2, 서미자2
소속 1경북테크노파크, 2(주)하늘호수
키워드 Schisandra chinensis; polyphenols; melanin; tyrosinase; B16 melanoma cells
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