| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 가을 (10/21 ~ 10/23, 일산 KINTEX) |
| 권호 |
21권 2호, p.1738 |
| 발표분야 |
생물화공 |
| 제목 |
Engineering Corynebacterium glutamicum to overproduce 1,5-diaminopentane |
| 초록 |
Biological production of 1,5-diaminopentane from renewable feedstock provides a promising and sustainable alternative to traditional petroleum-based chemical synthesis. In the present study, we report the development of a metabolically engineered Corynebacterium glutamicum strain that can produce 1,5-diaminopentane from glucose. By overexpressing L-lysine decarboxylase in an industrial L-lysine overproducer C. glutamicum (U2 strain), 1,5-diaminopentane was not produced whereas L-lysine was detected. The cadA gene was thus codon-optimized, which led to a production titer of 31.94 g/L of 1,5-diaminopentane without exogenous feeding of L-lysine by fed-batch cultivation. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012-C1AAA001-2012M1A2A2026556).] |
| 저자 |
이상엽1, Seok Hyun Park2, Ziwei Luo3
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| 소속 |
1MBEL, 2Dept. of Chemical and Biomolecular Engineering (BK21 Plus rogram), 3KAIST / BioInformatics Research Center |
| 키워드 |
Metabolic engineering; Corynebacterium glutamicum; 1; 5-diaminopentane
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| E-Mail |
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| 원문파일 |
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