화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2008년 봄 (04/23 ~ 04/25, 제주ICC)
권호 14권 1호, p.1118
발표분야 재료
제목 Effect of shape of silver nanoparticles as a colorimetric sensor for protein conformational changes
초록 Colloidal silver nanoparticles (AgNPs) with various shapes were prepared by the procedures described by Jiang et al. Cytochrome c (Cyt C) is covalently bound to the AgNPs surface by thiol group in the cystein 102 residue as a colorimetric sensor for conformational change. UV-VIS absorption spectroscopy was utilized to identify the changes in the optical properties of silver colloid for pHs, ranging from 2 to 10. And transmission electron microscopy results represented the morphologied changes of AgNPs - Cyt C conjugation which was correlated with pHs. For the quantitative analysis, conformational changes of Cyt C on AgNPs surface were measured by atomic force microscopy.
저자 박준수, 김대흠, 김영훈
소속 광운대
키워드 SPR; silver nanoparticle; colorimetric sensor
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