학회 |
한국화학공학회 |
학술대회 |
2008년 봄 (04/23 ~ 04/25, 제주ICC) |
권호 |
14권 1호, p.1118 |
발표분야 |
재료 |
제목 |
Effect of shape of silver nanoparticles as a colorimetric sensor for protein conformational changes |
초록 |
Colloidal silver nanoparticles (AgNPs) with various shapes were prepared by the procedures described by Jiang et al. Cytochrome c (Cyt C) is covalently bound to the AgNPs surface by thiol group in the cystein 102 residue as a colorimetric sensor for conformational change. UV-VIS absorption spectroscopy was utilized to identify the changes in the optical properties of silver colloid for pHs, ranging from 2 to 10. And transmission electron microscopy results represented the morphologied changes of AgNPs - Cyt C conjugation which was correlated with pHs. For the quantitative analysis, conformational changes of Cyt C on AgNPs surface were measured by atomic force microscopy. |
저자 |
박준수, 김대흠, 김영훈
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소속 |
광운대 |
키워드 |
SPR; silver nanoparticle; colorimetric sensor
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E-Mail |
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원문파일 |
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