| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 가을 (10/25 ~ 10/27, 대전컨벤션센터) |
| 권호 |
23권 2호, p.1748 |
| 발표분야 |
생물화공 |
| 제목 |
Development of Acyl-Homoserine Lactone Specific Binding DNA Aptamer |
| 초록 |
Quorum sensing is a system of cell–cell communication that allows bacteria to recognize information about cell density and regulate gene expression. Many bacteria release a variety of chemical signaling molecules called autoinducer (AI). Acyl homoserine lactone (AHL) is the most famous class of AI and is mainly made by Gram-negative bacteria. Although research on AI has been extensive, the method of detecting AI is limited and requires long time. New faster detection method are required to overcome the existing limitation. Aptamers are short single-stranded DNA or RNA that can bind to a variety of molecules with high affinity and specificity. It can maintain their structure in different condition and can be easily modified for variety of purpose. In this study, SELEX was conducted for 10 round to select AHL specific DNA aptamers. Finally, binding affinity of aptamer candidates were estimated by using SPR, and five aptamers with high affinity to AHL were selected. This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ012653)” Rural Development Administration Republic of Korea. |
| 저자 |
오인환1, 신우리1, Simranjeet Singh Sekhon1, 조성진1, 안지영1, 민지호2, 김양훈1
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| 소속 |
1충북대, 2전북대 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
